Cell engineering is a fundamental field of modern molecular biology that enables the generation of genetically modified mammalian cell lines for functional genomics, disease modeling, target validation, and preclinical research. Stable Knockout and Knock-in cell models provide powerful tools for investigating gene function, validating therapeutic targets, and accelerating the development of innovative therapeutic strategies.

We generate genetically modified mammalian cell lines using Lonza 4D-Nucleofector™ technology.

Our services include:

• Generation of stable Knockout cell lines for functional gene studies.
• Generation of Knock-in cell lines carrying disease-associated variants or fluorescent reporters (GFP, RFP, Luciferase).
• Transfection with client-provided plasmids or CRISPR/Cas9 RNP complexes.
• Complete turnkey development, including construct design, nucleofection, clonal isolation, antibiotic selection, and molecular validation.

Our laboratory combines advanced cell engineering with high-performance analytical technologies.

Cell Engineering
• Lonza 4D-Nucleofector™
• Sterile Class II Biosafety Cabinets
• Steri-Cult CO₂ Incubators

Cell Imaging
• Nikon Eclipse
• Olympus CKX
• Bio-Rad TruBlu

Genetic Validation
• Bio-Rad QX200 ddPCR
• Illumina iSeq 100 NGS

This integrated workflow enables efficient cell engineering together with comprehensive molecular validation.

Every generated cell line undergoes rigorous quality assessment throughout the development process.

Quality control includes:

• Clone selection
• Gene copy number analysis using ddPCR
• Editing site verification using NGS
• Sterility monitoring
• Mycoplasma testing
• Continuous environmental control during cell culture

Unlike conventional cell engineering laboratories, GeCure Solutions integrates cell engineering, molecular genetics, genome editing, NGS, and ddPCR within a single research platform. This comprehensive approach enables the development of reliable, fully validated research cell models that support academic research, biotechnology innovation, and preclinical development.